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- Rnascope Multiplex Fluorescent Reagent Kit V2 User Manual Pdf
- Rnascope Multiplex Fluorescent Reagent Kit V2
- Rnascope® Multiplex Fluorescent Reagent Kit V2
Rnascope Multiplex Fluorescent Reagent Kit V2 User Manual Pdf
A method for manual and automated multiplex RNAscope in situ hybridization and immunocytochemistry on cytospin samples. RNAscope 2.5 LS Reagent Kit-Red User Manual (ACD, UM-322150 RevA. For other regions, please check Catalog Numbers with your local lab supplier. ® RNAscope 2-Plex Detection Kit (Chromogenic) User Manual 9 10 ® RNAscope 2-Plex Detection Kit (Chromogenic) User Manual Chapter 2. Before You Begin 2 IMPORTANT! For Part 1, Sample Preparation and Pretreatment Guide for FFPE Tissue, see Catalog No.
Rnascope Multiplex Fluorescent Reagent Kit V2
Rnascope® Multiplex Fluorescent Reagent Kit V2
- Other sub-kits and directions for use, refer to the RNAscope® Fluorescent Multiplex Assay User Manual (320293-USM). The reagents have a shelf life of nine months from the bulk manufacture date when stored as indicated in the following table: Protease III & Protease IV Reagents (Cat. 322340) Reagent Quantity Storage.
- The BaseScope™ Reagent Kit v2-RED, is based on ACD’s patented signal amplification and background suppression technology. The assay uses a novel and proprietary method of in situ hybridization (ISH) to visualize single RNA molecules per cell in a multitude of sample types mounted on slides. The BaseScope™ Assay is used for the detection of short RNA targets (50-300 bases).
- Tissue was embedded in Surgipath Cryo-Gel (Leica, 39475237) and sectioned at 14 µm. RNAscope probe hybridization, amplification and immunostaining were performed following the protocol provided in the RNAscope Multiplex Fluorescent Reagent Kit v2 user manual (ACD). ACD designed the dusp6 probe used in this study. Following the final wash step.